Ash Barty dramatically abandoned her Internazionali d'Italia quarter-final against Coco Gauff on Friday due to a right arm injury.
The world number one was leading 6-4 2-1 in Rome when, with her left thigh also heavily strapped, she pulled out of the match following talks with a trainer.
Barty's decision was precautionary as she will begin her French Open campaign in a little over two weeks' time, bidding to regain the title she won two years ago.
The scare came just hours after world number three Simona Halep announced she tore a calf muscle this week, putting the Romanian's Roland Garros prospects in some doubt.
Providing an update after the match with Gauff, which was interrupted by a rain delay, Australian Barty said: "The pain was becoming too severe so it was important to listen to my body and do the right thing knowing we have a slam."
Gauff, who had hit 12 winners to 15 unforced errors prior to her opponent's withdrawal, will now compete in her first career WTA 1000 semi-final.
However, the 17-year-old was made to wait until Saturday to discover who she will face as the match between Iga Swiatek and Elina Svitolina was postponed due to wet weather.
Karolina Pliskova remained on course for a second Rome triumph in three years, after saving three match points to edge out Jelena Ostapenko.
Coco Gauff advances to her 1st WTA 1000 semifinal and first semifinal on red clay.— WTA Insider (@WTA_insider) May 14, 2021
Gauff will move into the Top 30 for the first time on Monday.
Before facing Barty in the quarterfinals, Gauff notched tough wins over Putintseva, Sakkari, and Sabalenka.#ibi21 pic.twitter.com/xivQ8qlR1l
The 2019 champion, who also finished runner-up last year, was 5-4 down in the final set when digging deep to hold off her 49th-ranked opponent.
The thrilling match went to a tie-break and Pliskova reeled off seven straight points after losing the opener to advance 4-6 7-5 7-6 (7-1).
"I'm just proud of how I fought," Pliskova said at her post-match news conference. "I'm happy that I stayed in there and fought until the end.
"We played last month, so I knew what was going to be happening. You can never get ready for some of the balls which she's playing."
Petra Martic stands between Pliskova and a place in the final for the third year running after the Croatian defeated Jessica Pegula 7-5 6-4.
Pegula eliminated Naomi Osaka earlier in the week but was second best against Martic, who progressed in one hour and 40 minutes.
Martic trailed 2-0 in the opening set before twice breaking Pegula to move 3-2 in front, but her opponent hit back to make it 5-5 in a tight contest.
However, Martic managed to break in the 11th game and served out the set, before showing her steeliness in the second to remain on course for a maiden WTA 1000 crown.