Brazil star Neymar hobbled out of training with Brazil ahead of their Group E clash with Costa Rica on Friday.
Neymar was absent from training on Monday, missing the session a day after Brazil drew 1-1 with Switzerland in their opening World Cup clash.
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The Paris Saint-Germain forward received some tough treatment from Switzerland, being fouled 10 times during the game, his first competitive start since February.
A fractured foot kept Neymar out of the end of PSG's season but he made a goalscoring return to action with a stunning solo strike against Croatia in a friendly at Anfield, and was also on target in a 3-0 warm-up victory against Austria.
Tite's men looked set to receive a boost with Neymar taking part in Tuesday's session.
But after around 10 minutes he limped away with a member of staff to receive some treatment after looking clearly uncomfortable on the field.
And just like that, Neymar is gone.
— Sacha Pisani (@Sachk0) June 19, 2018
He has just hobbled off. #BRA #WorldCup @OmnisportNews https://t.co/JxJD13LPi3