Ousmane Dembele was substituted just six minutes into Barcelona's LaLiga clash with Celta Vigo on Saturday, making him a doubt for the Champions League trip to Liverpool.
The France international pulled up early on and was replaced by Alex Collado, with the club later confirming he had suffered a hamstring problem that would be assessed on Sunday.
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Dembele's participation in Tuesday's semi-final second leg at Anfield will now be in question.
The 21-year-old came off the bench in the 3-0 first-leg win over Jurgen Klopp's side and squandered a glorious opportunity to add a fourth with almost the last kick of the game.
Ernesto Valverde named a much-changed starting XI for the trip to Vigo, with Lionel Messi and Luis Suarez among the players rested.
