Wigan Warriors have stood down Joel Tomkins for four weeks after he was shown being abusive to bar staff in a video that also featured brother and team-mate Sam.
The siblings appeared drunk as they were filmed arguing with employees of a pub, with Joel fined £10,000 in addition to his suspension and Sam incurring a £5,000 penalty from the club.
Joel appeared to barge his way behind the bar and was pushed away, before using explicit language towards one individual. The footage ended with the Tomkins brothers leaving the establishment.
The video emerged after Wigan's 23-0 Challenge Cup thrashing at the hands of Warrington Wolves on Saturday, though reports suggest the incident happened over a month ago.
"I was pretty disgusted at the video that I saw on Saturday evening," said Warriors executive director Kris Radlinski in a statement.
"Our players know and fully understand the role that they have to play within the community and to see two of our high profile, senior players behave in such a way, disappointed me greatly.
"They aren't just role models for the young kids in the town, they're role models for the younger players in the squad. It is impossible to put a strong culture in place if players you trust to drive those standards act in such a way.
"All players are allowed periods of downtime but know that they must act responsibly and in a manner fitting of a professional sportsman."
Wigan confirmed an internal investigation will take place "into the circumstances surrounding the incident", with Joel's long-term future at the club to be discussed. A move to Catalans Dragons has already been confirmed for Sam.
"Having spoken to both Joel and Sam this morning [Sunday] they both expressed shame and regret about their behaviour and the effects it has had on all of the individuals concerned," said Radlinski.
"Both of them are fully aware that they have disrespected the club and the town - and let themselves and their families down."