Manchester City goalkeeper Zack Steffen was one of the notable omissions from the United States' 26-man World Cup squad confirmed by head coach Gregg Berhalter on Wednesday.
Chelsea's Christian Pulisic, Juventus' Weston McKennie, Milan's Sergino Dest and Leeds United pair Brenden Aaronson and Tyler Adams were all included in the squad which is in Group B alongside Wales, England and Iran.
Steffen, who is on loan at Championship club Middlesbrough from City, missed out with Matt Turner, Sean Johnson and Ethan Horvath preferred as the three goalkeepers.
Fulham defender Tim Ream along with Turkey-based forward Haji Wright have also been included, while exciting 19-year-old striker Ricardo Pepi has missed out, along with Paul Arriola and Jordan Pefok.
"Final roster decisions are always difficult, and we appreciate everyone's contributions who helped us get to this point," Berhalter said. "We believe we have a talented group, a strong team spirit, and one that is ready to compete."
This year's event marks USA's return to the World Cup having failed to qualify for Russia 2018. Deandre Yedlin is the only member of their last World Cup squad from 2014 to be carried over in this group.
The United States face Wales in their opening Group B match on November 21, before taking on England and Iran.
USA squad: Ethan Horvath (Luton Town), Sean Johnson (New York City), Matt Turner (Arsenal); Cameron Carter-Vickers (Celtic), Sergino Dest (AC Milan), Aaron Long (New York Red Bulls), Shaq Moore (Nashville), Tim Ream (Fulham), Antonee Robinson (Fulham), Joe Scally (Borussia Monchengladbach), DeAndre Yedlin (Inter Miami), Walker Zimmerman (Nashville); Brenden Aaronson (Leeds United), Kellyn Acosta (LAFC), Tyler Adams (Leeds United), Luca de la Torre (Celta Vigo), Weston McKennie (Juventus), Yunus Musah (Valencia), Cristian Roldan (Seattle Sounders); Jesus Ferreira (FC Dallas), Jordan Morris (Seattle Sounders), Christian Pulisic (Chelsea), Gio Reyna (Borussia Dortmund), Josh Sargent (Norwich City), Tim Weah (Lille), Haji Wright (Antalyaspor)