Thomas Lemar and Hector Herrera are the latest Atletico Madrid players to test positive for coronavirus.
Atletico confirmed last week that Joao Felix and new signing Moussa Dembele returned positive test results in consecutive days, ruling them out of Monday's LaLiga visit of Celta Vigo.
Now their midfield and attacking options have been further depleted, with Herrera and Lemar immediately beginning periods of isolation.
A club statement read: "Our players Hector Herrera and Thomas Lemar underwent antigen tests prior to the match against Celta Vigo according to the LaLiga protocol, giving a positive result for COVID-19, which was later confirmed with the relevant tests PCR.
"Both players remain isolated in their respective homes, strictly complying with the recommendations of the health authorities and LaLiga protocol."
Atletico did not provide any further information regarding their respective conditions.
Lemar and Herrera could miss as many as three games, with Atletico facing Celta, Granada and Levante in the next 10 days.